ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2015, Vol. 46 ›› Issue (10): 1882-1890.doi: 10.11843/j.issn.0366-6964.2015.10.023

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The Influence of BPI Protein on Cytokines Level in Argali Hybrid Sheep Infected with Mycoplasma Ovipneumoniae

GUO Hai-ying1,SHEN Wen1,CHEN Dong-mei1,CHEN Kai-li2,ZHANG Xiao-li1,GAO Bao-de2,SUN Yan-ming1*   

  1. (1.College of Animal Science and Technology,Shihezi University,Shihezi 832003,China;2.College of Life Science,Shihezi University,Shihezi 832003,China)
  • Received:2015-01-26 Online:2015-10-23 Published:2015-10-23

Abstract:

The aim of this study was to analyze the immunological response of the recombinant bactericidal/permeability-increasing protein(rBPI) in Argali Hybrid Sheep(AHS) infected with Mycoplasma ovipneumoniae (MO). Six Bashibai Sheep(BS) was taken as group A,while 12 AHS were randomly divided into group B and C.All the sheep were artificially infected with MO.From the 4th day after infection,group C was treated with rBPI protein,150 mg•sheep-1•day-1,and group A and B with the same dose of placebo(Normal saline).The concentrations of IL-8,IL-10,TNF-α and IFN-γ from serum at different times were detected by ELISA.At the end of the test,all the sheep was slaughtered,and the therapeutic effect was evaluated with pathological section of their lung tissue.Results were as follows:In the early phase of infection,the levels of IL-8,TNF-α,IFN-γ were elevated,while IL-10 revealed a lower trend.On the 7th day post-infection(DPI),the concentration of IL-8 in group C was lower than group A(P<0.05).From the 7th to 21st DPI,IL-8 in group B was significantly higher than group A(P<0.01).On the 5th DPI,IL-10 in group C was higher than group A(P<0.05),but lower than group B(P<0.01).On the 14th to 21st DPI,IL-10 in group C was lower than group A(P<0.05),but higher than group B(P<0.05).On the 14th to 21st DPI,IFN-γ in group C was higher than group A(P<0.05).On the 14 th to 21st DPI,TNF-α in group C was higher than group A(P<0.05,P<0.01),and lower than group B(P<0.05).Histopathological scores of the A,B,C group were 9.4,19.9 and 12.8,respectively.The score of group B had significant difference with group A and C(P<0.05,P<0.05).The results showed that rBPI has some treatment effect on AHS infected with MO and a regulatory role on serum cytokines.

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